The separation of fructose from fructose-glucose syrup by using macroporous dihydroxyborylated polystyrene resin(MDPR) has been amplified to get fructose syrup with a high purity. The absorbing and eluting conditions has been optimized, i. e., the ratio of length to diameter of the column was reduced from 175: 1 to 40: 1, which was applied to CSEP chromatography separation system. By applying this system, the purity of the final separated fructose was 95% with a concentration of 35%, and the recovery ratio reached 90%. This kind of resin showed promise in industrial application.
