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酒向飞

作品数:4 被引量:15H指数:2
供职机构:北京大学药学院药剂学系更多>>
发文基金:国家教育部“985工程”更多>>
相关领域:医药卫生更多>>

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A high performance liquid chromatography method for the quantitative determination assay of sitagliptin in rat plasma and its application in pharmacokinetics study
2011年
A new high-performance liquid chromatography (HPLC) method for the quantitative determination of sitagliptin in rat plasma was developed and validated for pharmacokinetics study. The plasma was spiked with the internal standard (hydrocortisone, IS), treated with sodium hydroxide, and extracted with ethyl acetate. The extracted analyte was injected into an Agilent Zorbax Extend-C18 column (250 mm×4.6 mm, 4 μm) maintained at 30℃ and monitored at 267 nm. The mobile phase consisting of methanol-water (60:40, v/v, containing 10 mM Tris and 10 mM triethylamine) was titrated to pH 9.0 using 1 mol/L hydrochloric acid. The flow rate was 1.0 mL/min. The method showed high specificity. Calibration curves of the peak area ratio of each analyte/IS versus sitagliptin concentration were linear in the range of 0.75-100.0μg/mL (r〉0.9957). The lower limit of quantification (LLOQ) was 0.75 μg/mL. The intra-day and inter-day coefficient of variation was lower than 10%. The accuracy (relative recovery) at three levels was 105.3% (0.75 μg/mL), 99.8% (10.0 μg/mL) and 99.0% (100.0 μg/mL). The extraction recovery was 81.5%, 82.4% and 84.5% at the concentrations of 0.75, 10.0 and 100.0 μg/mL, respectively. The short-term, long-term, freeze-thaw storage stability of plasma samples and the stability of standard solutions were satisfactory. This HPLC method is suitable for determining the concentration of sitagliptin in rat plasma and it was applied to determine the concentration-time profiles of sitagliptin in rat plasma following oral administration of sitagliptin.
酒向飞尚德为陈烨李新刚万小蒙周田彦卢炜
关键词:SITAGLIPTINPHARMACOKINETICS
正交试验优选石杉碱甲脂质体的制备研究被引量:10
2009年
目的优化石杉碱甲脂质体的制备工艺和处方。方法采用薄膜分散结合硫酸铵梯度法制备石杉碱甲脂质体,以包封率为评价指标采用正交试验优化石杉碱甲脂质体的最优处方和工艺条件。结果石杉碱甲脂质体的最优处方工艺条件为:孵化温度为60℃,药脂比为1∶40,孵化时间为30 min。所得脂质体的包封率为87.02%以上。结论由最佳处方工艺条件制备的石杉碱甲脂质体包封率较高,包封率和体外释放稳定,重现性好。
张彦青解军波陈文倩邓晨辉酒向飞周田彦卢炜
关键词:正交试验高效液相色谱
Determination of metformin in diabetic rat plasma by an improved ion-pair high-performance liquid chromatography: application to a pharmacokinetic study被引量:1
2012年
An efficient and sensitive ion-pair HPLC-UV method using atenolol as internal standard (IS) was developed and validated for the determination of metformin in the plasma of diabetic rats. Plasma samples were deproteinated with 10% (v/v) perchloric acid. Separation was achieved on a UltimateTM AQ-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase (pH 5.05) composed of acetonitrile-water (31:69, v/v, containing 0.002 M sodium dodecyl sulfate, 0.0125 M potassium dihydrogen phosphate, 0.015 M triethylamine) at a flow rate of 1.0 mL/min. The calibration curve was linear (r〉0.994) between 7.5 and 4000 ng/mL. The lower limit of quantification (LLOQ) was 7.5 ng/mL. The precision was validated and the relative standard deviation was in the range of 1.87% to 15.70%; the accuracy was between 93.98%-106.89%. The mean recoveries were 95.40% and 95.31% for metformin and IS, respectively. The relative error (RE) of stability at different storage conditions was within ±9.00%. This method was used to determine the concentration-time profile of metformin in diabetic rat plasma following an oral administration of metformin at the dose of 10 mg/kg. Our results indicated that ion-pair HPLC-UV method using UltimateTM AQ-C18 column was effective for the pharmacokinetic studies of high polarity compounds like metformin.
陈烨李汉青许娇娇酒向飞邓晨辉李新刚李良徐小晴周田彦卢炜
关键词:METFORMINPHARMACOKINETICS
RP-HPLC同时测定William's E培养基中地塞米松、睾酮和6β羟基睾酮的含量被引量:4
2008年
目的建立同时测定William′sE培养基中地塞米松、睾酮和6β羟基睾酮含量的RP-HPLC检测方法,并用该方法研究地塞米松对大鼠原代肝细胞CYP3A的诱导作用。方法采用Agilent1100HPLC系统,Phenomenex色谱柱,以乙腈和磷酸溶液组成的流动相梯度洗脱分离,以酮康唑为内标,流速1.00mL.min-1,检测波长245nm,柱温20℃。结果地塞米松、睾酮和6β羟基睾酮分别在53~19291,161~29318和58~21091μg.L-1内呈线性关系;它们的日间和日内精密度均低于15%;地塞米松、睾酮和6β羟基睾酮的最小定量限分别为53.00,161.00和58.00μg.L-1。结论该方法简便、准确、可靠,可用于地塞米松作用于大鼠原代肝细胞体系后,地塞米松、CYP3A底物睾酮及其代谢产物6β羟基睾酮在William′sE培养基中的浓度测定。
李良管峥马鑫酒向飞卢炜
关键词:地塞米松睾酮大鼠原代肝细胞细胞色素P450
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