Grass leaved Sweetflag (Shi chang pu),a traditional Chinese drug, is dried rhizome of Acorus tatarinowii Schott. and the main active components are αand β asarone.In order to control the interior quality of the Grass leaved Sweetflag, the HPLC method was built to determine the Contents ofαand β asarone in Grass leaved Sweetflag. The method was superior to the GS/MS method.