[Objective] This study aimed to select microalgae species which are capable to effectively remove arsenic contamination from water under natural conditions. [Method] Four microalgae species [Chlorella sp. (zfsaia), Chlorella minata, Chlorella vulgaris and Selenastrum capricormulum] were used as experimental materials and cultured with six different concentrations of As (Ⅲ) (0.5, 1.0, 2.0, 5.0, 10.0, 20.0 mg/L). Biomass, chlorophyll a content and other physiological indicators were determined to investigate the arsenic tolerance and biosorption of four microalgae species. [Result] Chlorella sp. is sensitive to arsenic toxicity, its growth was inhibited when arsenic concentration exceeded 10 mg/L, with an EC 50 of 17.32 mg/L; when the arsenic concentration was 0-20 mg/L, growth of S.c, ww1 and C.v was not affected, which showed relatively high tolerance to arsenic, with arsenic removal rates of 77.02% , 72.18% and 81.36% respectively after 24 h. [Conclusion] This study indicates that microalgae have good application prospects for processing arsenic wastewater and being indicator plants of arsenic wastewater.