Four diterpenoid alkaloids were obtained form the roots of Aconitum kongboense Lauener.Among them, one is a new alkaloid, kongboensine (1), and the structure has been elucidated by spectral evidence.The others were identified as guayewuanine A (2), indaconitine (3) and talatisamine (4) respictevely.2 and 3 were isolated from this plant for the first time.