In this paper, the measurement of liquid mixing in a downcomer of segmental type of distillation column is presented. The extent of liquid mixing is calculated by means of a mixing pool model. The results indicate that liquid mixing in a downcomer is actually incomplete. It is a significant correction to the assumption of complete downcomer mixing or no downcomer mixing which is generally adopted in many distillation calculations. Besides, the present results are used in a two dimensional eddy diffusion model to calculate the distillation tray efficiency. It is shown that the assumption of complete downcomer mixing is closer to the actual situation than that of no downcomer mixing.