Capillary zone electrophoresis(CZE) has been used for the enantiomeric separation of isoprenaline.β cyclodextrin(β CD) and HP β CD were used as the chiral selectors and several factors influcing the separating resolution were discussed.
Cyclandelate was separated using a new derivative of mono 3 O phenylcarbamoyl β CD as chiral selector.It was discussed the effect of the concentration of chiral selector,pH,the concentration of buffer and organic modifier on the resolution.The results show that the chiral resolution(Rs)is strongly influenced by the concentrations of mono 3 O phenylcarbamoyl β CD and the pH of the background electrolyte.Under the same experimental conditions,cyclandelate cannot be separated by β CD and the commonly used HP β CD and DM β CD.This showed that the chiral separating ability of mono 3 O phenylcarbamoyl β CD is stronger.The recogition mechanism of mono 3 O phenylcarbamoyl β CD on these chiral drugs in the cyclodextrin modified capillary zone electrophoresis were discussed.
