Phytochemical investigations of Pseudolarix kaempferi Gord led to the isolation of 26 known compounds. They were identified as follows： pseudolaric acid D （1）, pseudolaric acid A （2）, pseudolaric acid B （3）, pseudolaric acid C （4）, oleana- 11,13（18） - dien-3β-O-acetyl （5）, oleana- 9（11）,12 -dien-3β-O-acetyl （6）, oleana- 11,13（18）-dien-3β-hydroxyl （7）, oleana- 9（11）,12 -dien-3β-hydroxyl （8）, celangulatin C （9）, celangulatin E （10）, 17β-tenacigenin B （11）, 11α-O-2-methyl butyryl-12β-O-acetyl tenacigenin B （12）, 11α-O-2-methyl butyryl-12β-O-tigloyl tenacigenin B （13）, β-sitosterol acetate （14）, umbelliferone （15）, 5,7-dihydroxyl coumarin （16）, xanthotoxin （17）, isopimpinellin （18）, formononetin （19）, calycosin （20）, cnidimol B （21）, thymine （22）, 3-furoic acid （23）, 2-furoic acid （24）, vanillic acid （25）, protocatechuic acid （26）. Compounds 5–24 were isolated from P. kaempferi for the first time. And the 1 H and 13 C NMR spectra of compound 1 were completely assigned for the first time.
From the aerial parts ofNauclea officinalis （Pierre ex Pitard） Merr. et Churl, one new indole alkaloid paratunamide E （1） has been isolated together with six known indole alkaloids, paratunamide A （2）, nauclealotide C （3）, nauclealotide A （4）, vincosamide （5）, strictosamide （6） and naucleamide F （7）. Naucleamide F （7） was isolated from Nauclea officinalis for the first time. All of the seven compounds above were elucidated by spectroscopic methods including 1 D and 2 D NMR soectroscoDic analyses.
One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8（14）-en-15-al （1） together with four known diterpenoids kirenol （2）, ent-2-oxo-15,16,19-trihydroxypimar-8（14）-ene （3）, darutigenol （4） and darutoside （5） were isolated from the ethanol extract of Siegesbeckia pubescens. The planar structures and relative configurations of these compounds were elucidated by comprehensive spectroscopic analysis.
Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedures were utilized on the aqueous solution after partitioned with ethyl acetate, which resulted in the separation of a new furostanol-type glycoside and four known compounds. The structures of the isolated compounds were elucidated on the basis of spectroscopic techniques（1D and 2D NMR, IR, HRESIMS） as 26-O-β-D-glucopyranosyl-（25S）-5β-furost-20（22）-en-1α,3β,26-triol-1-O-α-L-arabinopyranosyl-（1→2）-[α-Lrhamnopyranos-yl]-3-O-α-L-rhamnopyranoside（1）,（1β,3β,16β,22S）-cholest-5-en-1,3,16,22-tetrol-1,16-di-（β-D-glucopyranoside）（2）, diosgenin（3）, β-sitosterol（4）, ecdysterone（5）.
The aim of current study was to investigate the chemical constituents of the needles of Taxus media. The isolation and purification of components were achieved by a series of chromatography including silica gel, Sephadex LH-20, MCI and reversed- phase HPLC. Their structures were indentified based on 1D, 2D NMR, and mass spectral analysis. Seven taxane diterpenoids were isolated and identified as ll3,2tt,9ct,1013-tetrahydroxy-5ct-cinnamoyloxy-3,11-cyclotaxa-4（20）-en-13-one （1）, 2it, 7[3, 9ct, 1013, 13tt-pentaacetoxytaxa-4（20）, 11-dien-Sct-ol （2）, 2-deacetoxy-5-decinnamoyltaxinine J （3）, taxuspine F （4）, taxuspinanane K （5）, taxchin A （6）, and baccatin IV （7）. Among them, 1 is a new compound and compounds 2, 5, 6 and 7 are isolated from Taxus media for the first time.
