By using PEG 1500 glass chromatography column, 9 kinds of bactericidal substances (\%m\%\|hydroxybenzoic acid, methyl salicylate, catechol, acetic acid, acetaldehyde, acrylic acid, diethyl sulphide, borneol\|2 and camphanone\|2) and 9 kinds of aromatic substances (ethyl acetoacetate, allyl alcohol, citral, acetylmethylcarbinol, iso\|pentanol, borneol\|2, ethyl acetate, benzaldehyde and terpineol) were extracted out through the separation experiments on the volatile gas released from 12 main greenery tree species in Shanghai. Furthermore, the hygienical substances ingredient proportion between the different trees was measured. This experiment provides scientific basis for constructing healthy ecological greenland.