Cu-based H2S sorbents were studied by Temperature-Programmed Sulfiding(TPS) method. The effects of CuO contents, calcining temperature and supports on desulfurization performance were investigated. At low CuO content, Cuo could be self assembled as monolayer on supports because of the interaction between CuO and support. With the increment of CuO content, CuO phase appeared. At higher calcinting temperature the interaction Cuo with Al2O3 made the sulfiding temperature higher. The supports such as Al2O3, ZrO2, TiO2 and 13X zeolite play different roles in TPS processt sulfiding temperature changed with various sopports.