In order to investigate characteristics of nitrogen use efficiency of CMS line Rong 18A, four hybrid rice combinations （Rong18 You188, Rong18 You198, Rong18 You662, Rong18 You908） derived from CMS line Rong 18A were used as experimental materials with Ⅱ You838 and Gangyou725 as controls. A split-plot experimental design was performed with four nitrogen fertilizer application rates to analyze the effects of different nitrogen fertilizer application rates on the yield and yield component factors of rice. The results showed that nitrogen fertilizer could significantly increase effective panicle number to improve rice yield. The increment in rice yield per unit nitrogen fertilizer application rate demonstrated a descending trend with the increase of nitrogen fertilizer application rate. Under low nitrogen levels, hybrid rice combinations derived from Rong 18A exhibited relatively high nitrogen use efficiency and showed remarkably high nitrogen use efficiency under low nitrogen levels.
Rong 18A is an Indica three-line sterile line bred with M5B×D Xing B, and has the characteristics of stable sterility, high outcrossing rate, good combining ability and good quality. With Rong 18A as an experimental material and Ⅱ-32A, Jin 23A and Gang46 A as the CK materials, a seed setting rate estimation method by daily pollination was used for measuring its stigma vigor, to comparatively analyze the decreasing rates and lasting time of stigma vigor of various materials under natural condition. According to experimental results and combining with field breeding practice, the application of stigma vigor of sterile lines on hybrid rice seed production was discussed.
