With apical leaves of Lycium ruthenicum Murr as experimental material, the polyploids of L. ruthenicum were induced with colchicine solution, and total four polyploidy plants were identified by chromosome courts. The results showed that tetraploid plants of L. ruthenicum were successfully induced with 300 mg/L of colchicine solution after 7 and 21 d of induction, with 400 mg/L of colchicine solution after 14 days of induction, and with 500 mg/L of colchicine solution after 7 days of induction, respectively.
