A hydrogen isotope analytical method on individual n-alkanes in crude oil was established, including the sample pretreatments and the instrumental tests. The error of δD was less than 5‰. The factors which influenced determination, such as the effective separation between individual hydrocarbons, the organic H in target components completely convert into H2 and the accurate measurement by mass spectrometry, were discussed. The hydrogen isotope compositions and the geochemical significance about the individual n-alkanes of two type crude oils in Dongying City depression were also studied. The results show that the hydrogen isotope of individual n-alkanes is a good index sign for studying the paleoenvironment and the oil source.
