皖南尖吻蝮蛇蛇毒经分离和纯化得到出血毒素 (Aa H ) ,质谱测定分子量为 2 32 84.4± 0 .1。实验表明 ,色氨酸 (Trp)残基位于 Aa H 分子的疏水区。十二烷基磺酸钠 (SDS)和盐酸胍的加入使 Aa H 的分子构象发生变化 ,导致分子内 Trp残基的荧光淬灭。盐酸胍的加入使 Trp残基的荧光发射峰位明显红移 ,表明位于 Aa H 分子较疏水环境的 Trp残基相对外露。本文还就酸度、EDTA和金属离子对 Aa H 分子内
rac-Tetra（m-nitrobenzene）porphyrin, H2TNO2PP, synthesized from o-nitro-benzaldehyde and pyrrole was reduced to H2TamPP, from which tetra（o-phenylacetyl benzene） porphyrin and especially its α,α,α,α-isomer were obtained. The α,α,α,α-isomer was used as hapten to immunize BALB/C mice. The latter gave antibody value of 320 as determined by ELISA method, indicating that the artificial hapten could induce the production of antibody without use of carrier.
研究了几种淬灭剂对皖南尖吻蝮蛇蛇毒出血毒素 (Aa H )内源荧光的影响。溴代琥珀酰亚胺 (NBS)法测得每个 Aa H 分子中含有 3个 Trp残基。实验得到丙烯酰胺 (Acr)和 I-的碰撞淬灭常数 KSV分别为 38.3M-1和 4.2 7M-1,fa 分别为 0 .97和 0 .99,Acr和 I-均可以淬灭 Aa H 分子中几乎全部 Trp残基的荧光 ,初步验证 Acr与 Aa H 分子间无明显的相互作用。结合 Aa H 的荧光发射光谱可以推断 ,Aa H 中的 3个
The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their structures were determined by 400 MHz1H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The resultsof gel electrophoresis analysis gave the average molecular weight of 1. 27 ×10-22 kg for eachantigen. The average connected ratio of BSA to hapten was 1: 12. Other UV and fluores-cence spectral properties of the antigens are reported.
