A membrane-less constructed wetland microbial fuel cell (CW-MFC) is constructed and operated under continuous flow with a hydraulic retention time (HRT) of 2 d. Fed with glucose, the CW-MFC generates a stable current density of over 2 A/m3 with a resistor of 1 kΩ and has a chemical oxygen demand (COD) removal efficiency of more than 90% after the startup of 2 to 3 d. A series of systems with the electrode spacings of 10, 20, 30 and 40 cm are compared. It is found that the container with the electrode spacing of 20 cm gains the highest voltage of 560 mV, the highest power density of 0. 149 W/m 3, and the highest Coulombic efficiency of 0.313%. It also has the highest COD removal efficiency of 94. 9%. In addition, the dissolved oxygen (DO) concentrations are observed as the lowest level in the middle of all the CW-MFC reactors. The results show that the more COD is removed, the greater power is generated, and the relatively higher Coulombic efficiency will be achieved. The present study indicates that the CW-MFC process can be used as a cost-effective and environmentally friendly wastewater treatment with simultaneous power generation.