人工鱼礁(Artificial reefs,ARs)是一种有效的鱼类诱集设施和重要的渔业管理工具。本研究以科学引文索引(Science Citation Index Expanded,SCIE)数据库为基础,利用文献计量方法,对国际人工鱼礁研究文献的年代、主要作者关键词、研究内容以及发文国家主要建设情况等进行全面分析。分析结果表明,国际人工鱼礁研究年均产出论文数量不多,美国是人工鱼礁研究发文数量最多的国家。国际研究主题主要包括人工鱼礁区生物群落结构与环境因子的相关性研究、人工鱼礁生物诱集效果研究、工程学研究以及社会、经济效益研究,且各国研究各有侧重、各具特色,研究内容因建设目标而异。该研究结果可为我国人工鱼礁研究和建设提供参考依据。
本研究在于探索世界主要国家地区俯冲带研究论文产出情况,以期为我国俯冲带研究和相关决策制定提供参考。以Web of Science信息平台的科学引文索引(Science Citation Index Expanded,SCIE)数据库为数据源,利用文献计量方法,分析国际俯冲带研究的年度变化、期刊分布、主要研究国家或地区的发文影响力及合作情况、主要研究机构和国际热点研究内容。结果显示,国际俯冲带研究论文产量一直呈增长趋势;美国的论文产出数量最多、合作中心性最强;中国俯冲带研究论文近3年增量明显,ESI高被引论文比例最高,但国际合作中心性较弱,其发文最多的机构为中国科学院;俯冲带热结构、俯冲与地震的关系、俯冲带中的流体与岩浆作用以及俯冲带成矿是近年来的研究热点。
Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole genome. DNA PCR amplification after digestion with restriction endonuclease Sau 3A Ⅰ, and （CA）12, （GA）12 probes marked with biotin were used for microsateUite DNA enrichment. The product fragments were connected with carder pGEM-T and transferred into DH5α Escherichia coli competent cells, and radioactive isotope probes marked with γ^-32 p were used for the second hybridization. As a result, a total of 1600 bacteria were obtained in the microsatellite genomic libraries, positive clones accounted for 90.91% before hybridization and 81.25% after hybridization, amounting to 1300. One hundred and ninety-six positive clones were selected for sequencing, and 192 clones included microsateUite sequences. The microsateUite sequences obtained, mono-nucleotide, quad-nucleotide and quint-nucleotide repeat motifs were observed beside double-base-pairs CA/GT, GA/CT. Seventy primers were designed according to the flanking sequences by using software Primer Premier 5.0, and 32 primers were selected to be synthesized. After optimizing PCR reaction conditions, 28 primers were amplified and produced clear purpose bands. The aim of our research was to promote the development and utilization of E. reieherti genomic resource, and lay the foundation for optimizing E. reieherti breeding strain in order to detect the genetic diversity and construct a genetic map.
