The advances in the application of sterilizing techniques against pest rodents in China are introduced in the paper. The development of chemosterilants, improvements of botanic sterilants, production of new steroid hormone sterilants, introduction of immunosterility and excellent properties of the sterilants are outlined. The 揝pace Occupation Theory?of sterile techniques is advanced after practice. The botanic sterilants with gossypol and trichosanthin as its main agents were screened and successfully applied in the large area control in the northern forest area of China. The safety of sterilants to non-target animals such as rats, rabbits, dogs, monkeys and chickens was summarized.