In order to effectively conduct emotion recognition from spontaneous, non-prototypical and unsegmented speech so as to create a more natural human-machine interaction; a novel speech emotion recognition algorithm based on the combination of the emotional data field (EDF) and the ant colony search (ACS) strategy, called the EDF-ACS algorithm, is proposed. More specifically, the inter- relationship among the turn-based acoustic feature vectors of different labels are established by using the potential function in the EDF. To perform the spontaneous speech emotion recognition, the artificial colony is used to mimic the turn- based acoustic feature vectors. Then, the canonical ACS strategy is used to investigate the movement direction of each artificial ant in the EDF, which is regarded as the emotional label of the corresponding turn-based acoustic feature vector. The proposed EDF-ACS algorithm is evaluated on the continueous audio)'visual emotion challenge (AVEC) 2012 dataset, which contains the spontaneous, non-prototypical and unsegmented speech emotion data. The experimental results show that the proposed EDF-ACS algorithm outperforms the existing state-of-the-art algorithm in turn-based speech emotion recognition.