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李淑娟

作品数:5 被引量:37H指数:3
供职机构:云南农业大学农学与生物技术学院更多>>
发文基金:云南省自然科学基金更多>>
相关领域:农业科学生物学更多>>

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Correlation Analysis of Genetic Distance and Heterosis of the Major Brassica napus L. Cultivars in Yunnan Province被引量:1
2014年
[Objective] The correlation between genetic distance and heterosis of the major Brassica napus L. cultivars in Yunnan Province was analyzed. [Method] The genetic distances among the 8 major Brassica napus L. cultivars in Yunnan Province were investigated with the SSR molecular marker technique. Moreover, the correlation between genetic distance and field appearance of heterosis was ana-lyzed. [Result] There was a certain correlation between the genetic distance and heterosis of crossing parents (P〉0.05). [Conclusion] It is difficult to predict the het-erosis of Brassica napus L. cultivars and to screen parents by using the SSR molecular marker technique.
刘岱李淑娟张传利朱海平彭少丹周筱妍徐蕾林良斌
利用SSR标记研究铁皮石斛的遗传多样性被引量:21
2015年
本研究利用SSR分子标记技术,对36份来自云南、浙江、广东、广西等地的铁皮石斛进行遗传多样性分析。结果表明:共获得249个SSR标记位点,其中有242个标记位点具有多态性,多态位点百分率为97.19%。遗传多样性分析表明中国铁皮石斛品种具有较高的遗传多样性,其有效等位基因数为1.604,平均期望杂合度为(He)0.344,平均Shannon多样性指数为0.508,其中光明石斛2号与瑞丽石斛1号之间的遗传相似系数最大,也只有0.835 3,在遗传相似系数为0.662时,36份铁皮石斛分别被分成了三个类群。本研究利用大量居群来研究铁皮石斛的遗传多样性,进一步探求其亲缘关系,可为种质资源的保护与新品种的选育提供基础。
徐蕾刘莉彭少丹李晚谊张庭李智敏王翰墨李淑娟林良斌
关键词:铁皮石斛SSR
云南主栽甘蓝型油菜品种的遗传多样性分析与分子鉴定被引量:3
2014年
利用SSR分子标记技术,对9份来自云南的主栽甘蓝型油菜品种进行遗传多样性及其分子鉴定分析。共获得146个SSR标记位点,其中有119个标记位点具有多态性,多态率达81.5%。遗传多样性分析表明,云南主栽甘蓝型油菜品种具有较高的遗传多样性,其有效等位基因数为1.422 8,平均期望杂合度(He)为0.248 2,平均Shannon多样性指数为0.371 1,多态位点百分率为68.91%,花油8号与花油3号之间的遗传相似系数最大,也只有0.831 9,在遗传相似系数为0.72时,9份甘蓝型油菜分别被分成了4个类群,并获得了能够区分云南9个品种的分子标记CB 10364。
李淑娟刘岱孙桂林彭少丹陈升位王勤方林良斌
关键词:甘蓝型油菜分子标记
20种甘蓝型油菜抗根肿病资源的筛选被引量:5
2015年
模拟根肿病发病条件,采用菌土接种法进行盆栽试验,对20个甘蓝型油菜品种进行根肿病抗性鉴定。根据病情指数将20个甘蓝型油菜品种的根肿病抗性分为高抗、抗病、低感、感病和高感。结果显示:华杂9号、华双3号的病情指数分别为25.06,25.03,属高抗品种;云花油早熟1号、陕油8号的病情指数分别为34.6,36.92,属抗病品种;中双9号、花油5号、花油8号的病情指数分别为45.8,46.89,47.33,属低感品种;玉红油2号等5个品种属感病品种;宁油16号等8个品种属高感品种。因此,华杂9号、华双3号表现为较强的抗根肿病能力,可以作为油菜抗根肿病育种的材料。
高愿高峰李淑娟彭少丹陈升位林良斌
关键词:油菜根肿病抗性
Screening of Drought-tolerant Brassica napus L.Varieties and Analysis on Their Physiologic and Biochemical Variations under Drought Stress被引量:7
2014年
[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde (MDA), soluble sugar, soluble protein, and the activities of superoxide dismutase (SOD), catalase(CAT), peroxidase(POD) were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out: YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.
李淑娟程量彭少丹陈升位王勤方林良斌
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