[Objective] The ovary microstructure and the changes of the follicle during the development period of blue fox during the estrous period were studied.[Method] Ten left ovaries were collected from 10 female blue foxes during the estrous period. The histological structure of the ovary and follicular development process were observed by using optical microscope. And 30 primordial follicles, 30 primary follicles, 15 growing follicles, 15 vesicular follicle, 15 mature follicles and the oocytes in the follicles of different developing stages were measured and photographed by eyepiece micrometer and photomicrographic technique respectively. [Result] The ovary of blue fox consisted of germinal epithelium, tunica albuginea, cortex and medulla. The cor- tex, which was located at the periphery of the ovary, contained many interstitial cells and follicles at different developing stages. Medulla was located below the cortex, with a lot of blood vessels. The diameters of follicles at different developmental stages and oocytes had greater differences during the developmental period of follicle. The diameter of follicles was 45.45-974.55 μm and the diameter of oocytes was 30.23-147.27 μm. The zona pellucida appeared at the primary follicle stage. The atresia of follicles occurred at all the developmental stages of follicle and the main manifestations were the shrunk oocyte and karyon, and loose and degraded granular cells. [Conclusion] The research provided histological basis for revealing the reproductive mechanism of female blue fox.
为了探讨饲粮铜添加水平对育成前期水貂生长发育及血液生化指标的影响。试验以蛋氨酸螯合铜为铜源,在7组(每组10只)86日龄雄性美国短毛黑水貂基础饲粮(含铜26.68mg/kg)中添加不同水平的铜(0、210、220、230、240、250、300mg/kg)组成水貂饲粮,进行60天的饲养试验和消化代谢试验,通过对平均日增重(Average Daily Gain,ADG)、料重比(Feed/Gain,F/G)、脂肪消化率(Fat Digestibility,FD)、氮消化率(Nitrogen Digestibility,ND)、净蛋白质利用率(Net Protein Utilization,NPU)、氮生物学价值(Biological Value of Protein,BV)和血清碱性磷酸酶(Alkaline Phosphate,AKP)、碱性磷酸酶(AlkalinePhosphatase,ALP)、铜蓝蛋白(Ceruloplasmin,CER)、谷丙转氨酶(Alanine Aminotransferase,ALT)、Cu-Zu-SOD指标的测定分析。结果表明:当基础饲粮中铜添加量为210～240mg/kg时,平均日增重和血液生化指标呈升高趋势,在240mg/kg时,体增重最快,平均日增重最高为17.50g/d(P<0.05),超过240mg/kg时,平均日增重和血液生化指标呈降低趋势。以蛋氨酸螯合铜为铜源,在86～146日龄雄性美国短毛黑水貂基础饲粮(含铜26.68mg/kg)中铜的添加量为210～240mg/kg,可明显促进水貂的生长发育和血清酶活性,以240mg/kg时,体增重和平均日增重最高(P<0.05);超过240mg/kg可明显抑制水貂的生长发育和血清酶活性。
