To clarify the effects of mechanical sowing and transplanting on dry mat- ter production of middle-season hybrid rice, a two-factor split plot design was used to study the effects of different sowing and transplanting methods and their interac- tion with the seedling number per hill or seeding time on dry matter accumulation, distribution and transformation of F You 498, a middle-season hybrid rice variety, under field conditions in 2012 and 2013. The results showed that there was a marked effect of the sowing and transplanting methods and their interaction with the seedling number per hill or seeding time on dry matter accumulation, distribution and transformation. The total population dry matter accumulation of the treatments with mechanical direct seeding (MDS) and machine-based transplanting (MT) was lower than that of the treatment with traditional manual transplanting (TMT). How- ever, MDS had higher dry matter accumulation and accumulating rate in the joint- ing-earing stage,and maintained higher stem-sheath exportation, export rate and transformation than MT and TMT; MT had higher dry matter accumulation and ac- cumulating rate in the heading-maturity period than MDS and TMT. Moreover, the treatments with low seedling number per hill or early seeding enhanced the assimi- lation of dry matter after heading,the ratio of dry matter accumulation after earing to biomass yield and the contribution rate of dry matter accumulation after earing, and a reasonable early sowing was favorable to increase the harvest index of middle- season hybrid rice under mechanical sowing and transplanting conditions.