A novel method for determination of SudanⅠ in food by single scan polarography has been developed in this paper.It was found that a well-shape second derivation polarographic wave was observed at peak potential Ep-0.79V(vs.SCE)in the mediums of sodium tetraborate and ethanol.The peak current for the wave was proportional to concentration of Sudan I.The linear range was 0.1μg/mL^1.6μg/mL.The coefficient of correlation was 0.9983.The detection limit of the method was 0.025μg/mL.Its relative standard deviation(RSD) was ≤1.1%(n=6).Average recoveries of blank were over the range of 88.0%~101.0% and average recoveries of pepper samples were over the range of 91.0%~117.4%.The proposed method is rapid,simple,accurate and highly sensitive,which provided the new gist to improving and perfecting the method for determination of SudanⅠ,and possessed a significant practical value.