[Objective] The present experiment was conducted to study the effects of bamboo vinegar on seed germination and seedling growth of different flue-cured to- bacco varieties, so as to provide some references for its application in tobacco pro- duction. [Method] By using indoor culture dish method and greenhouse seedling- raising plate culture,four different concentrations of bamboo vinegar dilution (50, 100, 200 and 400 times)were designed and clean water was used as the control (CK). The germination indices of Yunyan 97, Guiyan 2, Jiucaiping 2 and Bina 1 were de- termined after seed germinating. The seedling growth indices of Yunyan 97 were investigated after 10 days of spaying bamboo vinegar on seedling leaves at the 3r~ time. [Result] Compared with CK, different bamboo vinegar dilutions could inhibit the seed germination and seedling growth of all flue-cured tobacco varieties. The seed germination rate and index, vigor index, whole length, root length, stem length and fresh weight of seedlings of all varieties presented decreasing trend to varying de- grees, moreover, most of them reached significant level. Compared with CK, spray- ing different concentrations of bamboo vinegar dilution at the seedling stage could improve the quality of seedlings. In all bamboo vinegar treatments, the plant height, the maximum leaf length and root volume under 100 times dilution treatment were the highest with 3.36 and 10.14 cm, and 0.92 ml, respectively. And the stem girth, dry weight and the maximum leaf width of seedlings under 400 times dilution treat- ment also showed the highest with 1.82 cm, 0.262 g and 5.74 cm, respectively. [Conclusion] Supplying different concentrations of bamboo vinegar solution on culture dish could inhibit the seed germination and seedling growth of flue-cured tobacco, while spraying 100-400 times bamboo vinegar solution to leaves could promote the seedling growth and improve the quality of Yunyan 97 to varying degrees.