A new Schiff base, 2 hydroxy 1 napthaldehyde 2 amino thiazole(HNATS) and its Cu(Ⅱ) complex was synthesized. The complex[Cu(Ⅱ) (HNATS) 2] could exhibit an efficient peroxitase like catalytic activity, and catalyze the coupling reaction of 2,4 dichlorophenol and 4 amino antipyrine with hydrogen peroxide. The effect of SDS on the catalytic activity of mimetic enzyme was studied. Based on the reaction, the determination of O ·- 2 by this new spectrophotometric method was developed. The proposed method was used to determine SOD activity of garlic, scallion and onion with satisfactory results.
