To compare the content of main active constituent in cordyceps from different forest region in China.The active constituent in the cordyceps was withdrawed by microwave method,the cordycepin and adenosine content were measured by HPLC,the cordyceps acid was measured by periodic acid sodium colorimetry method,the polysaccharides was measured by phenol-sulphuric acid method.Cordyceps militaris’s cordycepin content was higher than 20 mg·g-1 in cordyceps from two forest regions in Jiangsu Province,cordyceps acid content was the highest,and was 49.38 mg·g-1 in C.militaris from Mountain Funiu forest region in Henan Province;the total quantity of four kinds of active constituent in C.militaris from two Jiangsu forest regions were the highest,surpassed 100 mg·g-1.The results showed that the main active constituent content in C.militaris from different forest region was largely different,the active constituent content in C.militaris from two Jiangsu forest regions was relative higher than others.