Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO) is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair (bp) candidate fragment was amplified from pepper with primers derived from the ACO sequence (AJ011109) reported by Ernesto. The putative new gene was analyzed by bioinformatics tools.