The interaction between methylene blue(MB) and yeast RNA(yRNA) was studied by the cyclic voltammetry.The interaction was also testified by the UV-visual absorption spectrum.The result indicated that the interaction of MB and yRNA was electrostatic action in the CH3COOH-CH3COONa buffer solution(pH=5.6).And with the increase of the concentration of yRNA,the absorption peak intensity was weaker and weaker.An indirect electroanalytical method for determining yRNA was developed.The minished peak currents of MB were proportional to the concentration of yRNA in the range of 5.0×10-3mg/mL-3.0×10-2mg/mL,and the linear regression coefficient was 0.9976.
A novel fluorimetric method is developed for the rapid determination of ribonucleic acid(yRNA)with salicylic acid(SA)as a fluorescence probe.It is based on the fluorescence enhancing of SA in the presence of yRNA with maximum excitation and emission wavelength at 299nm and 413nm respectively.Variables influencing fluorimetric response of the interaction between SA and yRNA,such as pH value and reaction time,are explored and optimized.Under optimal conditions,the calibration graph for yRNA is linear over the range of 1.0×10-6mol/L～5.0×10-5mol/L.The disturbance experiment is also studied.The corresponding detection limit is 1.0×10-7mol/L.The method is more sensitive and selective than currently applied techniques.
