Cellulose tris(3,5 dimethylphenylcarbamate)(CDMPC) was prepared and coated onto an aminopropylated mesopore spherical silica gel. The final product was used as the chiral stationary phase in high performance liquid chromatography for enantioseparation. The enantiomers of Flamprop methyl were resoluted directly on this kind of chiral stationary phase(CSP) with a mixture of hexane and 2 propanol as a mobile phase. The effects of concentration of 2 propanol in the mobile phase on the retention and resolution were investigated. The structural features of the solutes that influence chiral separation and the chiral recognition mechanism and the eluted sequence were discussed.
The title compound was prepared in yield of 93.3% by reaction of phenyl isocyanate with 2-chloro-4-amino pyridine. The latter was obtained by nitrification, reduction of 2-chloropyridine-N-oxide. The best conditions of the reaction were given.
