The trehalose 6 phosphate synthase gene tps 1 was amplified from yeast S.cerevisiae AS2.1416 cDNA library by polymerase chain reaction.This 1.5 kb fragment was cloned into Pst I and Bam HI sites of pGEM T easy vector and the sequence of the gene indicated the cloned tps 1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S.cerevisiae S288C.
