Objective To compare the difference of clinical efficacy between acupuncture of lifting and pressing manipulation and conventional acupuncture in motor function recovery of hemiplegic upper limb after stroke, so as to provide referential support for clinical application. Method Fifty-nine patients with upper limb hemiplegia after stroke were selected and divided into group A (conventional acupuncture ± lifting and pressing manipulation, 30 cases} and group B (conventional acupuncture, 29 cases} according to random number table method. Upper limb motor function and activity of daily living of patients in the two groups were scored before and after treatment, and the results were analyzed and compared. Results The score of upper limb FugI-Meyer motor function of patients was 22.4 ± 2.83 and 32.97 ± 2.92 before and after treatment in group A, and was 19.89 ± 3.13 and 26.62 ± 3.31 before and after treatment in group B, and the difference between the two groups before and after treatment was significant (P〈0.01}. The score of Barthel index of patients was 60.67 ± 4.65 and 73.00 ± 3.82 before and after treatment in group A, and was 50.52 ± 5.15 and 60.17 ± 4.89 before and after treatment in group B, and the difference between the two groups before and after treatment was not statistcally significant {P〉0.05). Conclusion Lifting and pressing manipulation combined with conventional acupuncture can better improve upper limb motor function of upper limb paralysis of patients after stroke.