Various zirconia stationary phases were prepared by modification with di-dentate Lewis bases: dodecyl-N,N-diacetic acid（RNCC）, dodecyl-N-acetic acid-N-methyleneposphonic acid（RNCP）, dodecyl-N,N-dimethylenephosphonic acid（RNPP） and dodecencylsuccinic acid（RCC）. The color reaction of RNPP-ZrO2 and RNCP-ZrO2 with ammonium molybdate indicate there are free phosphonic groups on the surface of the stationary phases, and the FTIR spectra of RNCC-ZrO2 and RNCC-ZrO2 indicate there are free carboxyl groups. The RCC-ZrO2 is far more stable than RNCC-ZrO2 under alkali station. The results of color reaction, FTIR spectra and the stability test of stationary phases suggest that, due to the chelating effect, RNCC, RNCP and RNPP adsorb on zirconia with only one carboxyl or phosphonic group, however, RCC adsorb on zirconia with two carboxyl groups.
