Liver is one of the most important organs in energy metabolism. Most plasma apolipoproteins and endogenous lipids and lipoproteins are synthesized in the liver. It depends on the integrity of liver cellular function, which ensures homeostasis of lipid and lipoprotein metabolism. When liver cancer occurs, these processes are impaired and the plasma lipid and lipoprotein patterns may be changed. Liver cancer is the fifth common malignant tumor worldwide, and is closely related to the infections of hepatitis B virus (HBV) and hepatitis C virus (HCV). HBV and HCV infections are quite common in China and other Southeast Asian countries. In addition, liver cancer is often followed by a procession of chronic hepatitis or cirrhosis, so that hepatic function is damaged obviously on these bases, which may significantly influence lipid and lipoprotein metabolism in vivo. In this review we summarize the clinical significance of lipid and lipoprotein metabolism under liver cancer.
目的建立一种低成本、高效率、操作简便的单核苷酸多态性(SNP)检测技术。方法采用自行设计的"ShineRoar 探针",结合融解曲线技术检测目的基因 SNP。并根据其工作原理分别对肿瘤坏死因子受体Ⅱ(TNFRⅡ)和载脂蛋白 M(apoM)的基因多态性进行检测;同时用 DNA 测序技术鉴定 ShineRoar 探针技术的准确性。结果融解曲线分析结果显示,某一基因的野生型及突变型纯合子分别在2个不同的融解温度出现融解谷。TNFRⅡ基因第6外显子196位突变(ATG→AGG)所产生的 T 和 G 等位基因的融解温度分别为(52.84±0.75)℃和(58.38±0.61)℃;apoM T-778C 突变所产生的 T 和 C 等位基因的融解温度分别为(42.55±0.73)℃和(49.19±0.57)℃。一致性 Kappa 检验显示 ShineRoar 探针技术与 DNA 测序技术的 SNP 检测结果一致(Kappa=1,P=0.000)。结论ShineRoar 探针技术简单、快速、准确,适用于大批量基因分型的研究。
