Singlet oxygen（1O2） is widely considered to play a major role in photodynamic therapy（PDT）,and thus an increasing attention has been focused on the direct detection of 1O2 near-infrared luminescence around 1270 nm for PDT dosimetry.A new sensitive detection system is developed to directly measure the temporal and spectral resolved 1O2 luminescence spectra.The triplet state and 1O2 lifetimes of Rose Bengal as a model photosensitizer in different solvents are determined,and the obtained results agree well with the published data.Our detection system has the potential application in 1O2 luminescence-based PDT dosimetry.
Singlet oxygen(^(1)O_(2))is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in diferent biological systems and in particular,in photodynamic therapy(PDT).However,the quantifcation of^(1)O_(2) generation during in vitro and in vivo pho-tosensitization is still technically challenging.To address this problem,indirect and direct methods for^(1)O_(2)detection have been intensively studied.This review presents the available methods currently in use or under development for detecting and quantifying^(1)O_(2) generation during photosensitization.The advantages and limitations of each method will be presented.Moreover,the future trends in developing PDT-^(1)O_(2) dosimetry will be briefly discussed.
