Three eudesmane sesquiterpene lactones, namely artemivestinolides A-C （1-3）, along with three known 11-epimeric lactones （4-6）, were isolated from the aerial parts ofArtemisia vestim. Their structures were elucidated on the basis of extensive spectroscopic analyses （IR, HR-ESIMS, 1D NMR and 2D NMR）, and the absolute configurations were determined by single-crystal X-ray diffraction （with copper radiation）. Furthermore, in an in vitro assay, the three new compounds exhibited a moderate inhibition of the lipopolysaccharide （LPS）-induced nitric： oxide （NO） production in BV-2 microglial cells.
Shuai-Hua TianXing-Yun ChaiKe ZanKe-Wu ZengPeng-Fei Tu
Chemical constituents of the leaves of Artemisia argyi were investigated. By using a variety of chromatographic techniques and spectroscopic methods, six compounds were isolated and identified as follows： clemaphenol A （1）, aurantiamide acetate （2）, camelliagenin A （3）, japonica acid （4）, labd-13（E）-ene-8α,15-diol （5）, and 313-acetoxy-20-oxo-21-nordammaran-23-oic acid （6）. Among these products, compounds 1 and 3-6 were obtained from the genus Artemisia for the first time and compound 2 was firstly reported from the species. Additionally, compound 5 displayed an inhibitory effect against the lipopolysaccharide （LPS）-induced nitric oxide （NO） production in BV-2 microglial cells with an IC50 value of 6.68 μM.
A chemical investigation of the aerial parts ofArtemisia vestita Wall. led to the isolation of 12 known sesquiterpenes, including 2 furan-containing sesquiterpenoids and 10 eudesmane sesquiterpene lactones. Their structures were identified as negunfurol （1）, schensianol A （2）, artemine （3）, erivanin （4）, 1,5-diepi-artemin （5）, acetylartemin （6）, naphtho[1,2-b]furan-2（3H）-one, 6-（acetyloxy） decahydro-9a-hydroxy-3,Sa-dimethyl-9-methylene-（3S,3aS,5aS,6S,9aS, ghS） （7）, naphtho[1,2-b]furan-2（3H）-one, 6-（acetyloxy）- 3a,4,5,5a,6,7,8,9b-octahydro-8-hydroxy-3,Sa,9-trimethyl- （3S,3aS, SaR,6S, SS,9bS） （8）, isoerivanin （9）, harrelierin （10）, （11S）-1- oxoeudesm-4（14）-eno-13,6a-lactone （11）, 1-epi-dehydroisoeranin （12）, respectively. All of these compounds were isolated from Artemisia vestita for the first time, and compounds 1 and 2 were isolated from the genus Artemisia for the first time.
Nine lignans were isolated and purified by silica gel, ODS, Sephadex LH-20 column chromatographies, preparative HPLC and recrystallization from the aerial parts ofArtemisia absinthium L., and identified as follows： diayangambin （1）, sesartemin （2）, epiyangambin （3）, （＋）arborone （4）, （-）syringaresinol （5）, epiashchantin （6）, caruilignan C （7）, 7β-caruilignan C （8）, yangambin （9）. Compounds 4, 5, 7, 8 were isolated from this plant for the first time.
Fourteen known compounds were isolated from the aerial parts of Artemisia anomala and identified as follows： zhepiresinol （1）, （-）-lariciresinol （2）, ficusal （3）, medioresinol （4）, （＋）-syringaresinol （5）, 1β,2β-epoxy-3β,4α,10α-trihydroxyguaian- 6α,12-olide （6）, 5β,10α-dihydroxy-1α/-dehydroleucodin （7）, 3α-chloro-8α-acetoxy-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αHguai- 11 （13）-en- 12,6α-olide （8）, 13-O-desacetyleudesma-afraglaucolide （9）, 1,10-seco-4ξ-hydroxy-muurol-5-ene- 1,10-diketone （10）, 1α-hydroxyafraglaucolide（11）, aurantiamide （12）, indole-3-aldehyde （13）, loliolide （14）. All these compounds were isolated from this plant for the first time and compounds 1-4, 8, 10, 13 were obtained from genus Artemisia for the first time.
