为探究烟草种子萌发过程对低温胁迫的响应,对萌发1 d、2 d和3 d的种子分别进行2 d低温处理(LT1、LT2和LT3),分析不同萌发时期种子遇到低温的蛋白与多肽变化,分别以萌发2 d、3 d和4 d的种子为对照(GS2、GS3和GS4),形成3个组合GS2 vs LT1、GS3 vs LT2和GS4 vs LT3。结果显示,GS2 vs LT1、GS3 vs LT2和GS4 vs LT3的差异多肽集合的交集共计20个多肽,GO和KEGG富集分析发现3个组合的差异蛋白均与碳代谢、氨基酸生物合成相关;此外鉴定到8个参与到低温应答的多肽,其中Nitab4.5_0002257g0090多肽在3个组合中同时被鉴定到,可能在低温应答过程中存在重要的功能。本研究为后续挖掘多肽在低温应答过程的功能提供了研究基础。
[Objective] The aim was to explore the effect of amino acid fertilizer on the growth, development and quality of flue-cured tobacco. [Method] With Yunyan 87 as tested flue-cured tobacco varieties, three treatments （chemical fertilizer （control）, chemical fertilizer＋solid amino acid, chemical fertilizer＋liquid amino acid） were set up to study the effects of amino acid fertilizer on flue-cured tobacco fertilizer reducing and efficiency increasing by means of contrast in the same field. [Result] The flue-cured tobacco applied solid amino acid fertilizer had the best performances of overall agronomic traits and the highest yield, while liquid amino acid fertilizer was conductive to the top leaf opening, making the raw tobacco appearance best, and could increase the proportion of middle and high quality tobacco, gaining good economic benefits. The flue-cured tobacco treated by liquid amino acid fertilizer had the optimal ratio of total sugar to nicotine and disaccharide differences in tobacco leaves, and the best coordination of chemical composition. Compared with the contrel, tobacco treated by amino acid fertilizer had less irritating and more comfortable taste, and the tobacco treated by liquid amino acid had light miscellaneous gas, and the best overall performance of sensory quality. [Conclusion] Chemical fertilizer＋liquid amino acids is conductive to promote the growth and development of flue-cured tobacco and improve the quality of tobacco leaves.
