A laser-induced resonance light scattering (RLS) imaging method to determine heparin is described based on the high light scattering emission power of the aggregation species of heparin with α, β, γ, δ-tetra(4-trimethylaminoniumphenyl)prophyrin (TAPP) in solution. By imaging the light scattering signals of the aggregation species, we proposed the method to determine the heparin with a detection range of 0.02 - 0.6 μg/mL and the detection limit (3σ) of 1.3 ng/mL.
It was found that multi-walled carbon nanotubes (MWNTs) could catalyze the redox reaction between chlorauric acid (HAuCl4) and reductive drugs such as tetracycline hydrochloride (TC), producing gold nanoparticles (Au NPs). By measuring the plasmon resonance light scattering (PRLS) signals of the resulting Au NPs, tetracycline hydrochloride can be detected simply and rapidly with a linear range of 4―26 μmol/L, a correlated coefficient (r ) of 0.9955, and a limit of detection (3σ) of 6.0 nmol/L. This method has been successfully applied to the detection of tetracycline hydrochloride tablets in clinic with the recovery of 101.9% and that of fresh urine samples with the recovery of 98.3%―102.0%.
Detection of backscattering signals (BSS) generally suffers from the interference of reflected light, and it is hard to apply these signals for analytical purpose. Herein we provided an optical assembly, which effectively eliminated the interference of reflected light so that the scattering signals of analyte could be measured distinctly. With this assembly, chlorine in human urine could be detected with the limit of detection (LOD) of 2.0 ng/mL by measuring the enhanced BSS signals produced between the interactions of chlorine with silver nitrate.
Ke Jun TAN Yuan Fang LI Cheng Zhi HUANG Xue Lian LIU
