0引言有限元方法(Finite Element Method)随着电子计算机的发展而迅速发展起来的一种现代计算方法。它是50年代首先在连续体力学领域、动态特性分析中应用的一种有效的数值分析方法,随后很快广泛的应用于求解热传导、电磁场、流体力学等连续性问题。Sergey等人[1]曾基于有限元的方法做过气泡动力学方面的分析。本文基于有限元方法。
Dynamically tracking hundreds of individual pits is essential to determine whether there exist "hot spots" for the formation of clathrin-coated pits or if the pits formed randomly on the plasma membrane. We propose an automated approach to detect these particles based on an improved á trous wavelet transform decomposition with automatic threshold selection and post processing solution, and to track the dynamic process with a greedy algorithm. The results indicate that the detection method can successfully detect most particles in an image with accuracy of 98.61% and 97.65% for adaptor and clathrin images, respectively, and that the tracking algorithm can resolve merging and splitting issues encountered when analyzing dynamic, live-cell images of clathrin assemblies.
