＜正＞Fermentation technology had become a promising method to replace the traditional chemical tenchnology for b...
Haoli Zhou~(1,2),Yi Su~1,Yinhua Wan~1 (1.National Key Laboratory of Biochemical Engineering,Institute process of engineering,Chinese Academy of Science,Beijing,100190,China
Plant water sources were estimated by two or three compartment linear mixing models using hydrogen and oxygen isotope （δD and δ^18O） values of different components such as plant xylem water, precipitation and river water as well as soil water on the Tibetan Plateau in the summer of 2005. Four dominant species （Quercus aquifolioides, Pinus tabulaeformis, Salix rehderiana and Nitraria tangutorum） in three typical ecosystems （forest, shrub and desert） were investigated in this study. Stable isotope ratios of the summer precipitations and the soil water presented variations in spatial and temporal scales. δ^18O values of N. tangutorum xylem water were constant in the whole growth season and very similar to those of deep soil water. Water sources for all of the plants came from both precipitations and soil water. Plants switched rapidly among different water sources when environmental water conditions changed. Rainwater had different contributions to the plants, which was influenced by amounts of precipitation. The percentage of plant xylem water derived from rainwater rose with an increase in precipitation. Water sources for broad-leaved and coniferous species were different although they grew in the same environmental conditions. For example, the broad-leaved species Q. aquifolioides used mainly the water from deep soil, while 92.5% of xylem water of the coniferous species P. tabulaeformis was derived from rainwater during the growth season. The study will be helpful for us to fully understand responses of species on the Tibetan Plateau to changes in precipitation patterns, and to assess accurately changes of vegetation distribution in the future.
＜正＞In the filed of non-destructive testing method and geological survey,contact type ultrasonic wave from poin...
XiaLing ZONG~(1*),Qun ZHAO~1,Chao CHEN~2,Qun ZHAO~1 1SINOPEC Geophysical Research Institute,Nanjing 210014,China 2 State Key Laboratory of Mechanics and Control of Mechanical Structures. Nanjing University of Aeronautics and Astronautics,NanJing 210016,CHINA
Objective: To study the therapeutic effect and mechanisms of recombinant adenovirus Ad-p14ARF in hepatocel- lular carcinoma cell lines. Methods: Morphology and trypan blue assay were adopted to evaluate the proliferation of different liver cancer cells after Ad-p14ARF infection. Cell apoptosis was confirmed by detecting phosphatidylserine (PS) externaliza- tion with Annexin V/PI double staining. Western blotting assay analyzed the expression of related proteins. Subcutaneous tumor model of BEL7402 was established to evaluate the therapeutic ability of Ad-p14ARF. Results: Ad-p14ARF suppressed cell growth, proliferation and promoted cell apoptosis of cancer cell lines with different genetic background. Ad-p14ARF in- hibited growth of liver cancer cells (HepG2, BEL7402) in a dose-dependent manner. Ad-p14ARF leaded to overexpression of Bax and p21, which were the downstream regulating genes of p53. Ad-p14ARF suppressed tumor growth significantly in the experimental therapy in nude mice bearing subcutaneous tumor of BEL7402. Conclusion: P14ARF gene is a powerful tumor suppressor gene to be used in cancer gene therapy. It may play an important role in gene therapy against the malignancies in the future.
Haili QianHaifeng SongXueyan ZhangXiao LiangMing FuChen Lin
The Large Sky Area Multi-Object Fiber Spectroscopic Telescope （LAMOST） published its first data release （DR1） in 2013, which is currently the largest dataset of stellar spectra in the world. We combine the PASTEL catalog and SIMBAD radial velocities as a testing standard to validate stellar parameters （effec- tive temperature Tefr, surface gravity log g, metallicity [Fe/H] and radial velocity Vr） derived from DR1. Through cross-identification of the DR1 catalogs and the PASTEL catalog, we obtain a preliminary sample of 422 stars. After removal of stellar param- eter measurements from problematic spectra and applying effective temperature con- straints to the sample, we compare the stellar parameters from DR1 with those from PASTEL and SIMBAD to demonstrate that the DR1 results are reliable in restricted ranges of Tefr. We derive standard deviations of 110 K, 0.19 dex and 0.11 dex for Tell, log 9 and [Fe/H] respectively when Teff〈 8000 K, and 4.91 km s-1 for Vr when Teff 〈 10 000 K. Systematic errors are negligible except for those of Vr. In addition, metallicities in DR1 are systematically higher than those in PASTEL, in the range of PASTEL [Fe/H] 〈 -1.5.
