AIM: To investigate the chemical constituents and their biological activities of the aerial parts of Euphorbia tibetica. METHOD: Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated through the use of extensive spectroscopic techniques including 2D-NMR, the structures of compounds 5 and 6 were confirmed by single-crystal X-ray crystallography. Bioactivities of all the isolated compounds were evaluated by the MTT method on A549 and anti-angiogenesis assay. RESULTS: One new compound, methyl 4-epi-shikimate-3-O-gallate(1), together with twenty-three known constituents(2–24) were isolated from the aerial parts of E. tibetica. CONCLUSION: Compound 1 is new, and the other compounds were isolated from this plant for the first time. Compounds 5–7, 9, 11, 17, 18 and 20 exhibited inhibitory effects on the growth of human lung cancer cell A549 and compounds 5, 7, 12, 13, 17 and 19 showed anti-angiogenic effects in a zebrafish model.
YANG Da-SongHE Qiu-XiaYANG Yong-PingLIU Ke-ChunLI Xiao-Li
Three new minor prenylated flavonoids,named macadenanthins A–C(1–3),together with three known ones(4–6),were isolated from the twigs of Macaranga adenantha.Their structures were elucidated on the basis of extensive spectroscopic analysis including NMR,UV and MS.The prenyl moieties in compounds 1–3 were further modified by cyclization and hydroxylation.The new compounds were tested for their cytotoxicity against four cancer cell lines(MCF-7,Hep G2,Hela and P388)and showed IC50 values in the range of 13.76–22.27 lM.
Objective To study the chemical constituents from the aerial parts of Hypericum beanii. Methods Various chromatographic techniques were used to separate the constituents and their structures were elucidated on the basis of extensive spectroscopic interpretation. Results Fifteen compounds were isolated from the aerial parts of H. beanii. Their structures were identified as hyperbeanol E(1),(E)-linalool-1-oic acid(2),(4S,5R)-5-(4′-methyl-3′-pentenyl)-4-hydroxy-5-methyldihydrofuran-2-one(3), benzoic acid(4), 4-(3-O-3″)-3″-methylbutenyl-6-phenylpyran-2-one(5), 4-hydroxy-4a,7-dimethoxy-4,4a-dihydrodibenzo-p-dioxin-2(3H)-one(6), isoimperatorin(7), 2,3-dimethoxyxanthone(8), 3,4-dihydroxy-2-methoxyxanthone(9), osajaxanthone(10), nigrolineaxanthone F(11), hypercohone G(12), betulinic acid(13), oleanolic acid 3β-caffeate(14), and isoastilbin(15). Conclusion Compound 1 is a new menthane monoterpene derivative which owns an extra lactone ring. Compounds 2–7 and 10–15 are isolated from genus Hypericum Linn. for the first time and the other compounds are first obtained from the plants in H. beanii.
Phytochemical investigation of the aerial parts of Leonurus japonicus led to the isolation of one new labdane diterpenoid, leojaponin D(1) and two new ionone derivatives, leojaponones A and B(2 and 3),together with seven known diterpenoids(4–10). Their structures were elucidated by extensive 1D and2 D NMR spectroscopic data and by comparison with data reported in the literature. Selected isolates were evaluated their effects on Jurkat IL2 secretion.
