The polarization and absorption principle of corona virus in the electric field is presented. It is expressed by the mathematic differential equation based on the physical model. According to the parameters of the dipole moment of virus, the electric field force exerted by the external electric field can be calculated. According to the parameters of size, mass and elastic modular, etc., the moment of inertia of virus can be calculated. According to the viscosity of tissues, the resistant force when virus rotates can be calculated. According to the balance condition of relaxation polarization, when the drive force equals to the resistant force, the rotating or swaying frequency of virus can be calculated. According to the heat producing condition such as friction, the temperature rising of virus can be calculated. When the temperature exceeds a certain threshold, virus would be inactivated. Through the calculation, it is found that the movement type of the virus depends on the intensity and frequency of the external electric field, which are the effects of "intensity widows" and "frequency windows". It also gives approximate calculation of the temperature rising of the virus according to this model. The vitro experiment confirms the reasonability of this model. The electric fields of different densities of a certain frequency are applied to the solution of avian infectious bronchitis virus. Through the toxicity experiment of the SPF chicken embryo, it is found that the toxicity is decreased exceedingly with a certain intensity of the applied voltage. EID50 decreases from 6.70/0.2 mL to 2.66/0.2 mL. It is proved that the avian infectious bronchitis virus can be inactivated with a certain low frequency and low-density electric field.
A resistor-capacitor(RC)circuit model is proposed to study the effect of nanosecond pulsed electricfield on cells according to the structure and electrical parameters of cells.After a nanosecond step fieldhas been applied,the variation of voltages across cytomembrane and mitochondria membrane both in nor-mal and in malignant cells are studied with this model.The time for selectively targeting the mitochondriamembrane and malignant cell can be evaluated much easily with curves that show the variation of voltageacross each membrane with time.Ramp field is the typical field applied in electrobiology.The voltagesacross each membrane induced by ramp field are analyzed with this model.To selectively target the mito-chondria membrane,proper range of ramp slope is needed.It is relatively difficult to decide the range ofa slope to selectively affect the malignant cell.Under some conditions,such a range even does not exist.
本文研究了低频交变磁场对细胞作用的理论机理和实验结果。交变磁场和交变磁场感应的电场对运动离子产生电场力,加速离子的运行。基于该理论分析,设计了一系列的实验来验证假定的理论,实验采用两种肿瘤细胞系(HL-60 and SK-Hep-1)。将肿瘤细胞暴露于50Hz,20mT连续正弦磁场4天,每24小时检测上清液Na+和K+浓度。结果发现,照射组和对照组的Na+和K+浓度有显著变化,实验结果和理论分析相符。
