Objective:To study the immunologic function of dendritic cells(DCs) cultured in two kinds of hepatoma cell line's supernatant and the enhancing effects of carboxymethylpachymaran(CMP) on DCs.Methods:DCs were harvested after stimulation by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin(IL)-4 from umbilical cord blood using density-gradient centrifugation method. Cultured supernatant of two hepatoma cell lines(HepG2 and HepG2.2.15) were collected for condition medium(CM) according to a volume ratio of supernatant to incomplete RPMI-1640 medium,which was 3:1.CMP was dissolved in incomplete RPMI-1640 medium.Experimental groups were divided according to the culture medium,either CM or with CMP in it.DCs subsets CD83,CD86,CD1a,and d-related human leukocyte antigens(HLA-DR) were analyzed by flow cytometry.The proliferation ability of allogeneic T cells in mixed lymphocyte reaction(MLR) stimulated by DCs was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) analysis.IL-12p70,interferon-γ(IFN-γ),and nuclear factor k B(NF- k B) were detected by enzyme-linked immunosorbent assay analysis.Results:The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of CM were lower than those of normal group(P<0.01).Compared with the normal group,groups treated with CMP showed a higher expression level of DCs subsets,lymphocyte reproductive activity,as well as IL-12 and IFN-γsecretion levels.Groups treated with CMP also demonstrated higher levels of DCs phenotype expression and IL-12 and IFN-γsecretion in supernatant of MLR and higher lymphocyte reproductive activity compared with CM group(P<0.05).Compared with the normal group,the expression level of NF-k B in DCs nuclear was higher in CMP groups but lower in two CM groups(P<0.05).After CMP was added,the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added(P<0.05).However,there was no significant difference between the tw
Objective:To observe the anti-virus effects of andrographolide(AD) on the retinoic acid-inducible gene-l(RIG-l)-like receptors(RLRs) signaling pathway when immunological cells were infected with H1N1.Methods:Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation,and immunological cells were harvested after cytokines stimulation.Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line(16HBE).The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium(MTT) assay.After the virus infected cell model was established,AD was added into the medium as a treatment intervention.After 24-h co-culture,cell supernatant was collected for interferon gamma(IFN-γ) and inteiieukin-4(IL-4) enzyme-linked immunosorbent assay(ELISA) detection while immunological cells for real-time polymerase chain reaction(RT-PCR).Results:The optimal concentration of AD for anti-virus effect was 250 μg/mL IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus,RIG-I,IFN-β promoter stimulator-1(IPS-1),interferon regulatory factor(IRF)-γ,IRF-3 and nuclear transcription factor κB(NF-κB) mRNA levels increased significantly(P<0.05).When AD was added into co-culture medium,the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased,RIG-I,IPS-1,IRF-γ,IRF-3 and NFk B decreased significantly in each group with significant statistic differences(P<0.05).Conclusions:The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality.And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.
OBJECTIVE:To observe the effect of the Traditional Chinese Medicine,modified Taohongsiwu decoction,versus pyridoxine on patients with hand-foot syndrome(HFS)from capecitabine,sorafenib,and gefitinib chemotherapy for gastric,lung,breast,colon,or rectal cancer.Also,to compare quality of life of patients in each group.METHODS:Patients were assigned randomly to group A or B.Group A was given modifiedTaohongsiwu decoction to soak hands and feet for 30 min,once daily.Group B was given 100 mg pyridoxine orally,twice daily.After a 2-week treatment,thetherapeutic effect was assessed by observing three major symptoms,including pain,ulceration,and muscular atrophy.This was assessed with the HFS-14 questionnaire.RESULTS:Significant differences were observed between the two groups in pain relief,and improvement of daily life,walking,and interpersonal communication(P<0.01).No significant differences in driving ability or interpersonal relationships were found.After 2 weeks,the effective rate was 88.3%in group A,which was significantly higher than the50%in group B(P=0.00).CONCLUSION:Modified Taohongsiwu decoction is effective in the treatment of patients with HFS.It improves patients'quality of life according to the HFS-14.
