The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu /μL,the recombination rate was about 94%,the library capacity was 1.08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.
