Different temperatures and PEF packing treatments were carried out on postharvest Huanghua pear fruit to investigate their effects on fruit storability and the regulatory mechanism. LOX activity, O2- content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production changed with peaks in the ripening fruit at 20℃ and were inhibited by cold storage, incidence of fruit woolness and fruit decay were lightened as well. Low temperature combined with PEF packing (PEF1 and PEF2) treatments could further improve the fruit storability, maintain preferable quality. There was no significant difference between PEF1 and PEF2 both during cold storage at 1℃ and shelf life at 20℃. The recommended storage period of Huanghua fruit was two months at It and could be extended one month longer with PEF packing treatments.
DOU Shi-juan, CHEN Kun-song, LU Jun-liang and ZHENG Jin-tu(Laboratory of Fruit Molecular Physiology and Biotechnology , Zhejiang University , Hangzhou 310029 ,P. R. China
Kiwifruit (Actinidia deliciosa (A. Chev.) C. F. Liang et A. R. Ferguson cv. Bruno) was used toinvestigate the effects of acetylsalicylic acid (ASA, 1.0 mmol/L, pH 3.5) and ethylene (100 mL/L) treat-ments on changes at endogenous salicylic acid (SA) levels and other senescence-related factors duringfruit ripening and softening at 20 ℃. The level of endogenous SA in ripening fruits declined and a closerelationship was observed between the change at endogenous SA level and the rate of fruit ripening andsoftening. ASA treatment elevated SA level in the fruit, slowed down the increases in lipoxygenase (LOX)and allene oxide synthase (AOS) activities, decreased the O22-. production in the preclimacteric phase andthe early phase of ethylene climacteric rise, maintained the stability of cell membrane, inhibited ethylenebiosynthesis, postponed the onset of the ethylene climacteric, and delayed the process of fruit ripeningand softening. On the contrary, application of ethylene to ripening kiwifruit resulted at a lower SA level, anaccelerated increases in the activities of LOX and AOS and the rate of O22-. production, an elevated relativeelectric conductivity and an advanced onset of ethylene climacteric, and a quicker fruit ripening andsoftening. It is suggested that the effects of ASA on ripening kiwifruit can be attributed to its ability toscavenge O22-. and/or to maintain stability of cell membrane.