The tripe gene block(TGB)genes of Barley stripe mosaic virus China strain(BSMV-CH)were amplified from cDNA of BSMV-CH RNAβ(GenBank accession No:AY789694)by PCR with special primer pairs,and cloned into pMD18-T vector for sequencing.Analysis of the sequences showed that the full length of BSMV-CH TGB1,TGB2 and TGB3 were 1 539,396 and 468 bp,with deduced 512,131 and 155 amino acids,respectively.BSMV-CH TGB1 shared 94.1%-95.4% nucleotide identities and 91.0%-94.5% amino acid identities with that of other BSMV strains,BSMV-CH TGB2 shared 96.5%-97.2% nucleotide identities and 98.5%-99.2% amino acid identities with that of other BSMV strains,and BSMV-CH TGB3 shared 95.7%-96.6% nucleotide identities and 94.2%-96.8% amino acid identities with that of other BSMV strains.Phylogenetic tree based on the amino acid of Hordeiviruses TGB genes showed that BSMV-CH was relatively closed to CV17 in genetic relationship.Therefore,BSMV-CH was deduced to be a recombined strain of CV17 and CV42.
