采用超临界二氧化碳萃取法(SFE CO2 )提取的乌拉尔甘草种子油,用气相色谱质谱(GC MS)联用技术对其挥发性成分进行了分析,首次分离得到52个峰,确定了其中的16种化合物;采用峰面积归一化法计算各成分相对百分含量,所鉴定成分占总馏出峰面积的80. 85%。主要成分邻苯二甲酸二丁酯和β谷甾醇乙酸酯具有较多的生理活性,为进一步开发甘草种子油新药研究提供了依据。
Supersonic extraction process of glycyrrhizia polysaccharide(GPS) was examined by orthogonal experiments . The optimized supersonic extraction process was obtained and mathematical simulation based on the supersonic extraction was established. The optimized condition for extraction of GPS is as follows: supersonic treatment time is 3 5 h, supersonic treatment temperature is 81 ℃, liquid solid ratio is 10 mL/g, number of times of extraction is 1. Under the optimized condition, the extraction rate of GPS is 5 40%, purity is 39 95% and the relative deviation of repeated experiments is 2 54%.
Extraction and determination method of solanesol in waste tobacco leaves by combination of(ultrasonic) extraction and high performance liquid chromatography(HPLC) was demonstrated.Ultrasonic(extraction) showed advantage over the other methods.Thereby the conditions of ultrasonic extraction were(optimized).The results show that extraction yield is the highest under 80 kHz for 20 min with 80% ethanol at a ratio of V(liquid)/m(solid)=10 mL/g.
