In this paper, we constructed a novel bifunctional superoxide dismutase（SOD）/glutathione peroxi- dase（GPx） mimic, a selenium-, copper-containing 35-mer peptide conjugate（Se-Cu-35P） in which a three-amino acid linker（（31y-Asn-Gly） connects the C-terminus of 17-mer polypeptide SOD mimic with the N-terminus of 15-mer po- lypeptide GPx mimic. The SOD and GPx activities of Se-Cu-35P are two orders of magnitude lower than those of natural SOD and GPx, respectively. It provides a GPx activity 56-fold higher than Ebselen（a well-known GPx mimic）. The glutathione（GSH） binding constant is 5.6× 10^2 L.mol 1. Se-Cu-35P synergistically resists against the inactivation by H202 and protects the mitochondria from oxidative damage in a dose dependent manner. These results highlight the challenge of generating an efficient SOD/GPx synergism mimic. It could facilitate the studies of the cooperation of GPx and SOD and could be a potential therapeutic agent for the treatment of ROS-mediated diseases,
A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a gravity-induced flow injection chip（gFIC） was exploited to replace a pump.Also two charge coupled devices（CCDs） were used to detect HepG2 cells by SPRi and image processing,respectively.The results of two CCDs are associated.Protein A was used to modify the sensing surface.The inlet angle was carefully adjusted for the device to get an enhanced image.In the test,the contrast among cell solutions at different concentrations can be easily distinguished.The other CCD using image processing can tell false-positive in some degree.This detection is label-free,real time,and precise.
Nowadays nucleic acid tests are promising to be considered as point-of-care testing（POCT）. However, no such devices are currently available that can perform all the functions, including absolute nucleic acid determination, worldwide on-site detection, rapid analysis and real-time results reporting via ubiquitous mobile networks simultaneously with full package and automated means of measurement. In this study, we presented a compact low-cost portable POC automated testing platform with all attributes mentioned above. A disposable self-priming compartmentalization（SPC） microfluidic chip is used to conduct isothermal amplification. The platform also includes a micro-computer controlled heating unit, an inexpensive optical imaging setup, and a mobile device with customized software. It may become a useful tool for the rapid on-site detection of infectious diseases as well as other pathogens.
