To investigate the dynamic change of heat shock protein 90(Hsp90) in the genesis and development of tumor, we successfully established tumor animal model using Marek's disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzymelinked immunosorbent assay(ELISA) method. Our result showed that Hsp90 location in the tumor tissue was significantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most significantly increased with the development of tumor. This is the first report to show the presence of Hsp90 in tumor tissues induced by the Marek's disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.
[Objective] The paper was to investigate the relationship between pathological lesion and transcription,expression and distribution of heat shock protein 60( HSP60) in kidney of chickens infected by Marek's disease virus( MDV). [Method] Tumor animal model was successfully established with chickens infected by MDV. Real-time RT-PCR,histopathological and immunohistochemistrical methods were used to detect the pathological lesion,transcription level,expression level and distribution of HSP60 in the kidney. [Result]Obvious pathological changes appeared in kidney tissue of chicken after injection of MDV for 21 d. HSP60 was mainly distributed in the cytoplasm of tumor cell and interstitial macrophages.The expression of HSP60 in challenge group was always higher than blank control group and vaccine control group,and the difference reached extremely significant level from 28 to 86 day-ages( P <0. 01); the expression of HSP60 in challenge group at 28 day-age was about 8. 608 and 12. 752 times of blank control group and vaccine control group,respectively. The mRNA transcription levels of HSP60 in challenge group showed a downward parabola during 7 to 42 day-ages; the mRNA transcription level of HSP60 in challenge group was rising in early stage( 7- 21 d),and then gradually dropped; it reached the peak value at 21 d,being 1. 222 and 1. 179 times of blank control group and vaccine control group,respectively.The mRNA transcription level of HSP60 was higher than two control groups throughout the entire experiment,and the difference reached extremely significant level from 14 to 28 day-ages( P <0. 01). [Conclusion] HSP60 expression in kidney tissue and its mRNA transcription level can reflect MDV infection,tumor formation and development progress,with close relationship with pathogenesis of tumor diseases,which is expected to be used as one of the determination indicators for diagnosis and morbidity process of tumor diseases in chicken.
