Cystic tumour of the atrioventricular node is a rare primary cardiac tumour that can cause complete heart block and sudden death. Here, we describe a male case aged 42 years who suddenly died without a medical and family history of cardiac illnesses. After detailed macroscopic and microscopic examinations, a cystic mass was found in the atrioventricular nodal region. The small lesion was less than 1 cm in diameter, and consisted of small and large cystic spaces and tubular structures lined by flat, cuboidal or squamous epithelium. Immunohistochemical staining revealed the tumour epithelium positive for epithelial membrane antigen, carcinoembryonic antigen, antigen epitopes AEI/AE3, cytokeratins CK5/6 and CK7, but negative for calretinin, HBME-1, Wilms＇ tumor 1, factor VⅢ, chromogranin, synaptophysin or smooth muscle actin, suggesting an endodermal rather than mesothelial origin.
PAN YunCHEN Ji-liangLI Zheng-jinZHAO Li-xianWANG MinLI Yao-kangTIAN Lin-boGUO Hua
目的分析艾滋病相关性弥漫大B细胞淋巴瘤(HIV-DLBCL)及艾滋病患者淋巴结反应性增生(HIV-RH)的microRNA表达谱,筛选差异表达microRNA,为研究microRNA在HIV-DLBCL发生发展及诊断治疗中的作用提供依据。方法收集筛选6例HIV-DLBCL及4例HIV-RH石蜡包埋组织标本,提取总RNA,进行microRNA芯片杂交,运用SPSS 17.0软件进行统计学分析;利用miRWalk、Targetscan、miRanda软件对两组中存在统计学差异的microRNA进行靶基因预测,并进一步通过Gene Ontology(GO)和Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库分析靶基因功能。结果与HIV-RH组相比,miR-4492、miR-208b-3p、miR-4738-3p、miR-4674在HIV-DLBCL组织中显著上调,miR-4453、miR-363-3p显著下调(表达变化>5倍)。预测6个靶基因可能与HIV-DLBCL的发生发展有关:miR-208b-3p的靶基因CDKN1A、NLK、SMAD4及miR-363-3p的靶基因E2F3、FOXG1、TBX3。结论筛选得到多个HIV-DLBCL中具有深入研究价值的microRNA及其靶基因,为进一步研究差异表达microRNA在HIV-DLBCL发病机制中的作用及其靶基因的生物学效应奠定了基础。
