‘Shinseiki’ pear, a superior cultivars of Pyrus pyrifolia, was bred from a cross of ‘Nijisseiki’× ‘Chojuro’ in Japan. In China, many excellent pear cultivars have been released using ‘Shinseiki’ as a parent. In this study, the S-genotypes of 13 progenies of ‘Shinseiki’ were identified by PCR-RFLP analysis, DNA sequencing and bioinformatic method. As a control, ‘Shinseiki’ was also included. PCR amplification showed that a common fragment of about 370 bp was generated in all the 13 cultivars. Restriction digestion analysis showed that PCR product amplified from ten cultivars ‘Huangguan’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinya’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’ and ‘Qinghua’ could be cut by S4-allele-specific endonuclease, and that from ‘Xinhang’, ‘Cuiguan’ and ‘Zaomeisu’ could be cut by S3-allele-specific endonuclease. DNA sequencing and bioinformatic analysis revealed that the undigested PCR fragment of ‘Huangguan’, ‘Cuiguan’, ‘Zaomeisu’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinhang’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’, ‘Xinya’ and ‘Qinghua’ was identical to S 16 , S5, S 35 , S 17 , S 16 , S1, S3, S1, S 31 , S 35 , S7, S 17 and S1, respectively. Consequently, the S-genotypes of 13 cultivars were as follows: ‘Huangguan’ （S4S 16 ）, ‘Yaqing’ （S4S 17 ）, ‘Xinhang’ （S1S3）, ‘Bishanerhao’ （S4S 16 ）, ‘Xinya’ （S4S 17 ）, ‘Cuilv’ （S3S4）, ‘Xizilv’ （S1S4）, ‘Zhonglierhao’ （S4S 31 ）, ‘Zhongliyihao’ （S4S 35 ）, ‘Zaomeisu’ （S3S 35 ）, ‘Cuiguan’ （S3S5）, ‘Qingxiang’ （S4S7） and ‘Qinghua’ （S1S4）.
