The protective enzyme(SOD,POD,CAT) activities and soluble protein content of pistil after pollination were compared between interspecific-cross and self-cross of cultivar,using Tulipa specie(T.tianschanica) from Xinjiang and T.gesneriana cultivars(’Kees Nelis’,’Negrita’) as test materials.The results showed that all the SOD,POD,CAT activities and soluble protein content changed after interspecific and self pollination,which means that the recognition between pollen and stigma can be initiated effectively when pollen fell on stigma despite of genetic relationship.Fluctuant changes were observed in 5 hours after pollination for protective enzyme activities and soluble protein content,and the fluctuation tendencies were different between interspecific and self pollination,as well as between ’Kees Nelis’ ×T.tianschanica and ’Negrita’×T.tianschanica.Self pollination appeared peaks of POD and CAT activities preceding interspecific pollination.Self pollination showed a larger rise and maintained rise for longer time than interspecific pollination.The SOD activity of ’Kees Nelis’ ×T.tianschanica changed more than that of ’Negrita’×T.tianschanica,meanwhile,the soluble protein content of the former declined preceding that of the latter.These differences were probably attributed to genetic relationship.
用8个RAPD引物对10个郁金香栽培品种和4个新疆野生种的群体进行了RAPD—PCR扩增，共扩增出38个有效位点，各位点谱带大小0．2～1．5kb，其中4个野生物种位点多态性为50％～68％、Nei’s基因多样性（H）为0．18～0．21、Shannon’s信息指数（I）为0．27～0．32；栽培种为元性系纯系，群体内部没有多态性片断，但各品种之间差异明显。聚类分析表明：供试群体可分为4大遗传类型，第一类为野生类型，包括4个野生物种，和其他国外栽培种之间遗传距离较远；第二类为单瓣晚花系列的栽培种Mrs J T Seheepers和Haleru，与野生种之间的遗传距离相对较近，因而与其有较好的杂交亲和潜力；第三类为栽培品种Negrita，具有独特的红紫花，与栽培种和野生种的平均遗传距离近乎相等，似乎是它们的居间类型；其它栽培品种为第四类；这些研究结果为郁金香培育奠定技术基础。
